## Description

ENCODE region-wide location analysis of H3 and H4 histones was conducted employing ChIP-chip using chromatin extracted from GM06990 (lymphoblastoid), K562 (myeloid leukemia-derived), HeLaS3 (cervix carcinoma), HFL-1 (embryonic lung fibroblast), MOLT-4 (lymphoblastic leukemia), and PTR8 cells. Experiments were conducted with antibodies to the following histones: H3K4me1, H3K4me2, H3K4me3, H3K9me3, H3K27me3, H3K36me3, H3K79me3, H3ac, H4ac, and CTCF.

Histone methylation and acetylation serves as a stable genomic imprint that regulates gene expression and other epigenetic phenomena. These histones are found in transcriptionally active domains called euchromatin.

## Display Conventions and Configuration

This annotation follows the display conventions for composite "wiggle" tracks. The subtracks within this annotation may be configured in a variety of ways to highlight different aspects of the displayed data. The graphical configuration options are shown at the top of the track description page, followed by a list of subtracks. To display only selected subtracks, uncheck the boxes next to the tracks you wish to hide. For more information about the graphical configuration options, click the Graph configuration help link.

## Methods

Chromatin from the cell line was cross-linked with 1% formaldehyde, precipitated with antibody binding to the histone, and sheared and hybridized to the Sanger ENCODE3.1.1 DNA microarray. DNA was not amplified prior to hybridization.

The raw and transformed data files reflect fold enrichment over background, averaged over six replicates.

## Verification

There are six replicates: two technical replicates (immunoprecipitations) for each of the three biological replicates (cell cultures).

Raw and transformed (averaged) data can be downloaded from the Wellcome Trust Sanger Institute via the ENCODE data access web site or the ENCODE FTP site.

## Credits

The data for this track were generated by the ENCODE investigators at the Wellcome Trust Sanger Institute, Hinxton, UK.