## Description Serial Analysis of Gene Expression (SAGE) is a quantative measurement gene expression. Data is presented for every cluster contained in the browser window and the selected cluster name is highlighted in the table. All data are from the repository at the SageMap project built on UniGene version Hs 159. Selecting the UniGene cluster name will display SageMap's page for that cluster. ## Methods SAGE counts are produced by sequencing small "tags" of DNA believed to be associated with a gene. These tags are produced by attatching poly-A RNA to oligo-dT beads. After synthesis of double stranded cDNA transcripts are cleaved by an anchoring enzyme (usually NIaIII). Then small tags are produced by ligation with a linker containing a type IIS restriction enzyme site and cleavage with the tagging enzyme (usually BsmFI). The tags are then concatenated together and sequenced. The frequency of each tag is counted and used to infer expression level of transcripts that can be matched to that tag. ## Credits All SAGE data presented here was mapped to UniGene transcripts by the SageMap project at NCBI.