## Description and Methods

This track shows gene annotations from the Vertebrate Genome Annotation (Vega) database.

The following information is excerpted from the Vertebrate Genome Annotation home page:

"The Vega database is designed to be a central repository for high-quality, frequently updated manual annotation of different vertebrate finished genome sequence. Vega attempts to present consistent high-quality curation of the published chromosome sequences. Finished genomic sequence is analyzed on a clone-by-clone basis using a combination of similarity searches against DNA and protein databases as well as a series of ab initio gene predictions (GENSCAN, Fgenes). The annotation is based on supporting evidence only."

In this assembly Vega annotations are shown only on chromosomes 1-25. The Vega annotation consists of BAC and PAC clones that were sequenced for the zebrafish clone mapping and sequencing project. This is based on the tiling path information from the FPC database.

## Display Conventions and Configuration

This track follows the display conventions for gene prediction tracks using the following color scheme to indicate the status of the gene annotation:

- Known (Dark blue): genes that are identical to known zebrafish complementary DNA or protein sequences and have an entry in the species-specific model organism database, ZFIN.
- Novel (Dark blue): genes that have an open reading frame (ORF) and are identical or homologous to zebrafish cDNAs, zebrafish ESTs, or proteins in all species. Novel transcripts are genes that fit the criteria of novel genes with the exception that an unambiguous ORF cannot be assigned.
- Putative (Medium blue): genes whose sequences are identical or homologous to zebrafish ESTs but do not contain an ORF.
- Predicted (Light blue): genes based on ab initio prediction and for which at least one exon is supported by biological data (unspliced ESTs, protein sequence similarity with mouse or tetraodon genomes, or expression data from Rosetta).
- Unclassified (Gray).

The details pages show only the Vega gene type and not the transcript type. A single gene can have more than one transcript which can belong to different classes, so the gene as a whole is classified according to the transcript with the "highest" level of classification. Transcript type (and other details) may be found by clicking on the transcript identifier which forms the outside link to the Vega transcript details page. Further information on the gene and transcript classification may be found here.

## Credits

Thanks to Kerstin Howe, Mario Caccamo and Ian Sealy at the Wellcome Trust Sanger Institute for providing the GFF files and additional annotations for the Vega genes. Vega gene annotations are generated by manual annotation by the Zebrafish sequence analysis group and the HAVANA group at the Sanger Institute and ZFIN.