## Description

Target protein Pol II (Covance 8WG16) was purified by chromatin immunoprecipitation from HeLaS3 (cervix epithelial adenocarcinoma) cells. The series is comprised of three biological replicates, done using ChIP-Sequencing on a Solexa machine. DNA fragments are selected for sizes in the range of 100-300 bps, then 27nts tags are sequenced from the ends of these fragments.

## Methods

The sequenced tags are mapped onto the hg18 build of the human genome using the Eland application (Illumina). Only tags that map uniquely with at most two mismatches are included. 7,478,694 tags were mapped uniquely to the genome from the combined data from the three biological replicates.

87,253 Pol II binding sites were determined using a peak threshold of 7 overlapping mapped fragments which corresponds to an expected false discovery rate of 0.1% as estimated by computer simulation.