## Description

This track contains tBLASTn alignments of the peptides from the predicted and known genes identified in the hg16 Known Genes track as of 21 July 2004.

## Methods

The predicted proteins from the human knownGene track were aligned with the human genome using the tblastn. The proteins were obtained by merging annotation data from the kgXref table with the sequence and name found in the knownGenePep table. This resulted in a set of 40,115 protein sequences. The tblastn results were filtered using a threshold E-value of 1e-100 and, for each known gene peptide query, the top 5 hits to the $organism genome were kept.

## Credits

tBLASTn is part of the NCBI Blast tool set. For more information on Blast, see Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403-410.

The remaining required utilities were written by Jim Kent, Brian Raney, Rachel Harte and Heather Trumbower. This track was produced in collaboration with the Zebrafish Genome Initiative at Childrens Hospital, Boston.