## Description

This track shows conserved mammalian microRNA regulatory target sites in the 3' UTR regions of Refseq Genes, as predicted by TargetScanS.

## Method

Putative miRNA binding sites on mRNAs were identified using six-nucleotide seed sequences from all known miRNAs conserved among human, mouse, rat, dog and chicken. These seed sequences were probed against the 3' end of mRNAs conserved over the five genomes. For further details of the methods used to generate this annotation, see Lewis et al. (2005).

## Credit

Thanks to Benjamin Lewis at MIT and the Whitehead Institute for providing this annotation. Additional information on microRNA target prediction is available on the TargetScan website.

## References

Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 2005 Jan 14;120(1):15-20.