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Sanger Studio

Inspect chromatograms, primer mapping, trimming, overlap assembly and QC decisions.

Connecting…
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Read length and consensus length are different checks. The default minimum usable primer read is 300 bp; the default minimum final sequence is 800 bp. A 700 bp read can contribute to a longer assembled consensus when it passes read QC and overlaps its partner read. A read that fails read-level quality filters is excluded from the assembly.

Select an isolate

Load a Mailroom map or select AB1 files from Browse. The table below lets you verify and correct each read’s primer and orientation before assembly.
Local-only interface · uses BranchManager Paper Trail/Merge Meeting · no project database is read or changed